Molecular EpidemiologySequence analysis of genes encoding major antigenic determinantsTo characterise the ASFV isolates circulating in the field, sequences from genes encoding p72, p30 and p54 will be obtained from strains collected in the epidemiological studies. Based on these data, phylogenetic trees are constructed and virus isolates can be placed into broad groups. Analysis of variable genome regionsThe stability of DNA virus genomes means it is likely that genome wide screening methods will have to be used to distinguish closely related virus isolates rather than sequence analysis of one or two gene coding region. Preliminary work has been carried out on four genome regions which contain arrays of tandem repeats. These are within the coding regions or three genes, 9-RL, J268L, and KP86R an d in one intergenic region the BtSj fragment. Once a standard protocol has been established, centralisation of data of the various studies will allow the investigation of the spread of infection and the attributing sources of infection. |
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